A description of these two strains and the case history of the patient are given in this report. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Vibrio cholerae cholera vibrio vulnificus vibrio parahaemolyticus vibrio alginolyticus plesiomonas choleras. Tof mass spectrometry for the rapid identification of vibrio v. Vibrio fluvialis is a halophilic gramnegative bacterium. Vibrio fluvialis and gastroenteritis annals of internal. Considering recent increase in numbers of diarrheal outbreaks and sporadic extraintestinal cases, v. Vibrio fluvialis is an emerging foodborne pathogen of increasing public health concern. Vibrio fluvialis is a halophilic, polarlyflagellated, gramnegative bacterium. It has a straight to slightly curved rod cell morphology that is motile by means of polar flagella. Concomitant bacteremia caused by vibrio fluvialis and. We describe a case of concomitant vibrio fluvialis and v. Vibrio fluvialis cases have symptoms of vomiting and diarrhoea, frequently with bloody stools. Download fulltext pdf download fulltext pdf bioremediation of mercury by vibrio fluvialis screened from industrial effluents article pdf available in biomed research international 20177.
By creating a small, but smart library of the amine transaminase from vibrio fluvialis, we were able to shift the acceptordonor spectrum towards aldehydes bearing a c 4 c 5 aliphatic side chain. Vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. However, using molecular tools, it is easy to cholreae v. Speciesspecific identification of vibrio fluvialis by pcr targeted to the conserved transcriptional activation and variable membrane tether regions of the toxr gene. In this work, we investigated the underlying genomic properties and. Strains formerly classified as the aerogenic gasproducing biogroup of vibrio fluvialis were shown by dnarelatedness to be a separate species. Download selected download selected concept code concept name preferred concept name. We created isogenic nontoxigenic mutants to assess. Frontiers functional characterization and conditional. It was first isolated in 1975 from the stool of a diarrhea patient in bahrain and categorized as group fvibrio and rechristened as vibrio fluvialis in 1981 8. Development of a quadruplex loopmediated isothermal. Vibrio fluvialis is an important foodborne pathogen that causes diarrheal illness and sometimes extraintestinal infections in humans. Photobacterium damsela is a halophilic marine vibrio found in tropical and semitropical aquatic environments.
Antimicrobial resistance scan for sep 15, 2016 cidrap. Vibrio fluvialis in patients with diarrhea, kolkata, india. The virulence phenotypes and molecular epidemiological. Upon discovery, this organism was considered to be similar to both vibrio and aeromonas species, but was ultimately determined to. Vibrio fluvialis, an estuarinebased halophilic vibrio, causes opportunistic infections in humans resulting in bloody diarrhea. Evolutionary model of cluster divergence of the emergent. Illnesses occurred year round, with peaks in spring and autumn. Is vibrio fluvialis emerging as a pathogen with epidemic potential in coastal region of eastern india following cyclone aila. This is, to our knowledge, the first report of this type of wound infection. Vibrio fluvialis and vibrio furnissii isolated from a. Authors of over 50 institutions that have signed a publish and read agreement with the society are now eligible for fee free open access. Engineering the amine transaminase from vibrio fluvialis towards branched.
Vibrio fluvialis is considered to be an emerging foodborne pathogen and has been becoming a high. Vibrio furnissii in the blood is rarely reported, which may explain why clinical features of bloodstream infections with this organism have not been described. The mechanisms that contribute to the bacterial survival and disease are still poorly understood. Four residues of the active site pocket three hydrophobic. Vibrio parahaemolyticus, vibrio vulnificus, vibrio alginolyticus, nono.
Four sets of speciesspecific primers were designed with different restriction sites contained in the inner primers. We describe here a novel method for measuring in vitro antibody secretion from the tissue culture of human b lymphocytes in peripheral blood mononuclear cells pbmc after oral vaccination with a killed cholera vaccine. Extraintestinal infections by vibrio alginolyticus and vibrio metscnikovii have also been reported occasionally. Vibrio fluvialis is a waterborne bacterium first isolated from patients with severe diarrhoea in bahrain in the 1970s by a. We describe a patient who developed skin lesions and v. In this study, we sequenced the genome of a clinical v.
Bioremediation of mercury by vibrio fluvialis screened from industrial effluents kailasamsaranya,1 arumugamsundaramanickam,1 sudhanshushekhar,1 sankaranswaminathan,2 andthangavelbalasubramanian1 1casinmarinebiology,facultyofmarinesciences,annamalaiuniversity,parangipettai608502,india. Researcharticle bioremediation of mercury by vibrio fluvialis screened from industrial effluents kailasamsaranya,1 arumugamsundaramanickam,1 sudhanshushekhar,1 sankaranswaminathan,2 andthangavelbalasubramanian1 1casinmarinebiology,facultyofmarinesciences,annamalaiuniversity,parangipettai608502,india 2avccollegeofartsandscience,mannampandal,mayiladuthurai609305,india. Patients experience diarrhoea, abdominal pain, fever and. A quadruplex loopmediated isothermal amplification lamp method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. Vibrio fluvialis and vibrio furnissii isolated from a stool sample of one patient.
Vibrio fluvialis, vibrio furnissii, and vibrio hollisae cause gastroenteritis to human, whereas vibrio damsella is a pathogen of extraintestinal infection. The distribution of virulence factors, microbiological and molecular epidemiological features of v. Value set details centers for disease control and prevention. Vibrio alginolyticus, vibrio fluvialis, and vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal dna similar to several vibrio cholerae heme iron utilization genes. Vibrio fluvialis is a pathogen commonly found in coastal environs. Bioremediation of mercury by vibrio fluvialis screened. A 46yearold man was admitted to our hospital with acute gastroenteritis, which began 36 hours after he ingested raw oysters. Vibrio furnissii formerly aerogenic biogroup of vibrio fluvialis, a new species isolated from human feces and the environment november 1983 journal of clinical microbiology 184. I know you said you were not keen on giving me an anaesthetic and taking the tooth out yourself all at the same time.
This hemolysin was purified to homogeneity by using sequential hydrophobicinteraction chromatography with phenylsepharose cl4b and gel filtration with sephacryl s200. Find, read and cite all the research you need on researchgate. The medium contains 1% peptone plus 4% sodium chloride and 5 micrograms of novobiocin per ml, ph 8. Enzymelinked immunosorbent assay elisa titers of the antibody secreted in the cell supernatant were determined. Broth medium for enrichment of vibrio fluvialis from the. Vibrio species are free living bacteria that are commonly found in aquatic environments.
The virulence phenotypes and molecular epidemiological characteristics of vibrio fluvialis in china pu liang, xiaoying cui, xiaoli du, biao kan and weili liang abstract background. Pdf vibrio furnissii formerly aerogenic biogroup of. Comparison of the heme iron utilization systems of. Identification of genetic bases of vibrio fluvialis. Though this pathogen can be easily isolated by existing culture methods, its identification is still a challenging problem due to close phenotypic resemblance either with. Outbreaks have been linked to the consumption of raw oysters, and shrimps. We found that the closest relationship was between v. Rapid identification and characterization of vibrio. Genetic studies on vibrio fluvialis and its enterotoxins. The distribution of virulence factors and molecular epidemiological features of this pathogen remain mostly unknown. Researchers reported the gene in 27 vibrio fluvialis isolates in kolkata, india. Vibrio fluvialis is considered to be an emerging foodborne pathogen and has been becoming a high human public health hazard all over the world, especially in coastal areas of developing countries and regions with poor sanitation.
A medium was designed for the enrichment and enumeration of vibrio fluvialis from environmental samples. Speciesspecific identification of vibrio fluvialis by pcr. It has a molecular weight of 63,000 and an isoelectric point of 4. The halophilic bacterium vibrio fluvialis is an enteric pathogen that produces an extracellular hemolysin. Vibrio furnissii formerly aerogenic biogroup of vibrio. We examined clinical and epidemiological features of 575 laboratoryconfirmed cases of vibrio gastroenteritis in alabama, florida, louisiana, and texas from 1988 to 1997 the us gulf of mexico regional vibrio surveillance system. Health, general cholera toxin health aspects diarrhea genes infection. Vibrio vulnificus, an opportunistic pathogen, is the causative agent of a lifethreatening septicemia and a rising problem for aquaculture worldwide. It has the potential to cause outbreaks and has an association with paediatric diarrhoea.
Three variants allowed the asymmetric synthesis of 2,2. We write to report the case of a man who developed enteritis due to vibrio fluvialis after eating raw seafood. Engineering the amine transaminase from vibrio fluvialis. Strains formerly classified as the aerogenic gasproducing biogroup of vibrio fluvialis were shown by dna relatedness to be a separate species. It is associated with severe infections of wounds acquired in warm coastal areas. The israeli journal of aquaculture bamidgeh xxx, 20xx. Vibrio gastroenteritis in the us gulf of mexico region. A small subset of these species can cause disease in humans, including gastroenteritis, wound infections, and septicemia in susceptible hosts. Vibrio fluvialis and vibrio furnissii have been associated with diarrhea but have rarely been isolated in the united states. In other bacterial species, type vi secretion systems t6sss are known to contribute to bacterial pathogenicity by exerting toxic effects on host cells or competing bacterial species.
Though this pathogen can be easily isolated by existing culture methods, its identification is still a challenging problem due to close phenotypic resemblance either. Development of a novel in vitro assay als assay for. It is a sodium chloriderequiring, oxidasepositive, nitratepositive organism that ferments dglucose and other carbohydrates with the production of acid and gas. Furthermore, clinical strains have emerged from every clade of v. Furniss and his colleagues, and is considered to be an emerging pathogen with the potential to have a significant impact on public health. The genetic factors that differentiate its clinical and environmental strains remain enigmatic. Ndm1 detected in 27 vibrio fluvialis isolates in india. Vibrio fluvialis hemorrhagic cellulitis and cerebritis. The pai can be acquired by horizontal gene transfer and contains genes for several virulence factors. A high index of suspicion is required for diagnosis of this specific pathogen and concordant infection. Alteration of the donoracceptor spectrum of the samine. We describe a case of vibrio fluvialis hemorrhagic cellulitis and cerebritis following multiple fireant stings and wading in brackish water.
Hickmanbrenner fw, brenner dj, steigerwalt ag, schreiber m, holmberg sd, baldy lm, lewis cs, pickens nm, farmer jj. Mukhopadhyay 1, masahiro asakura 2, shinji yamasaki 2, s. Vibrio fluvialis is a halophilic vibrio species associated with acute diarrhoeal illness in humans. Lippincott journals subscribers, use your username or email along with your password to log in. Vibrio fluvialis causes choleralike bloody diarrhoea, wound infection and primary septicaemia prevalent in individuals with a weakened immune system worldwide. The organism produces an enterotoxin et, a cytotoxin ct, and a cytolysin cl. Ata with a widened binding pocket, as exemplified for a range of ketones. Although mcr1 has often garnered drugresistance headlines of late, an earlier gene that also confers antimicrobial resistance, ndm1 new delhi metallobetalactamase has not gone away, as evidenced by a study yesterday in emerging infectious diseases. Cytotoxic and cell vacuolating activity of vibrio fluvialis isolated from paediatric patients with diarrhoea. A critical role for hemolysin in vibrio fluvialisinduced. Speciesspecific identification of vibrio fluvialis by pcr targeted to the conserved transcriptional activation and variable membrane tether regions of the toxr gene rupa chakraborty 1, sutapa sinha 1, asish k. View the article pdf and any associated supplements and figures for a period of 48 hours.